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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene continues to be observed in several tumors, which ends up in chromosome instability, and so may add to tumorigenesis. This protein was observed to affiliate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential function in selling cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have been described. Two further splice variants had been reported but in-depth nucleotide sequence info is just not yet out there.
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Blocking peptides are peptides that bind specifically into the target antibody and block antibody binding. These peptide typically includes the epitope recognized by the antibody.
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This tutorial outlines further achievable optimization of cellular and immunoassay parameters to be sure the very best success are...
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This manual outlines even further possible optimization of cellular and immunoassay parameters to make certain the very best success are...
Antibodies bound to the blocking peptide not bind on the epitope to the concentrate on protein. By evaluating the staining with the blocked antibody vs . the antibody by itself, you can see which staining is distinct.
Focus on Facts Cyclin E belongs to your highly conserved cyclin spouse and children, whose members are characterized by a spectacular periodicity in protein abundance from the cell cycle. Cyclins purpose as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which lead towards the temporal coordination of every mitotic event. Cyclin E varieties a posh with and features for a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S changeover. This protein accumulates on the G1-S period boundary and is degraded as cells progress by S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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